Application

Anti-TRAP-1 Antibody, clone TR-1 is an antibody against TRAP-1 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation.

Research CategorySignaling

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TRAP-1 in normal human bladder and human bladder cancer tissue.
Western Blotting Analysis: A representative lot detected TRAP-1 primarily in the inner mitochondrial membrane (IMM) and mitochondrial matrix (Mx), but not the outer mitochondrial membrane (OMM) or the intermembrane space (IMS) of fractionated mitochondria preparations from HeLa cells (Yoshida, S., et al. (2013). Proc. Natl. Acad. Sci. U. S. A. 110(17):E1604-E1612).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Heat shock protein 75 kDa, mitochondrial (UniProt Q12931; also known as HSP 75, TNFR-associated protein 1, TRAP-1, Tumor necrosis factor type 1 receptor-associated protein) is encoded by the TRAP1 (also known as HSP75) gene (Gene ID 10131) in human. The mammalian HSP70 family consists of the mitochondrial HSP75 (TRAP-1), the endoplasmic HSP78, the cytoplasmic HSC70 (a.k.a. HSC73 and HSP73) and its inducible counterpart HSP72 (a.k.a. HSP70). TRAP-1 regulates a metabolic switch between oxidative phosphorylation and aerobic glycolysis. TRAP1-deficiency results in enhanced mitochondrial respiration and fatty acid oxidation, as well as increased cellular accumulation of tricarboxylic acid cycle intermediates, ATP, and reactive oxygen species species. TRAP-1-deficient cells display suppressed glucose metabolism and strikingly enhanced invasiveness, implicating a tumor suppressor role of TRAP-1.

Immunogen

Recombinant protein corresponding to human TRAP-1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TRAP-1 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~75 kDa observed